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Discovering the Genome

Discovering
the Genome

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Course Modules

  • What Is Genomics? Why Do Genomics?
  • Tour of the Genome
  • DNA Sequencing
  • RNA Sequencing
    • Why Look at RNA Instead of DNA?
    • Measuring RNA
    • Sequencing RNA
    • Preparing and Sequencing RNA
      • Fragmenting RNA
      • Converting RNA into cDNA
      • Making a Second Strand of DNA
      • Attaching DNA Adapters
      • Performing Sequencing Reaction
    • Activity: RNA-seq Data on the GTEx Portal
  • What Makes a Nerve Cell Different from a Skin Cell?
  • Bioinformatics: What? Why? Who?
  • RNA Sequencing: Up Close with the Data
  • Browsing Genomes
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  • RNA Sequencing/
  • Preparing and Sequencing RNA

Converting RNA into cDNA

Next, we must convert the RNA into DNA. We use an enzyme called "reverse transcriptase" to create a complementary DNA (cDNA) sequence from the RNA fragment. This creates hybrid molecules that are a combination of RNA and cDNA.

 

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Discovering the Genome

The High School Genomics Project at the
University of Pennsylvania
c/o Nano/Bio Interface Center
3231 Walnut St, Rm 112C
Philadelphia, PA 19104

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