Why Look at RNA Instead of DNA?
If we want to compare the functions of two different kinds of cells in your body, like muscle cells and skin cells, should we look at their RNA or DNA content?
Which technique would you use if you are trying to discover new genes: microarray or RNA-seq?
RNA-seq. With microarrays, we need to know which genes we want to measure before we perform the experiment.
Preparing and Sequencing RNA
What are the four steps in RNA-seq library preparation?
Step 1: Fragment RNA.
Step 2: Convert RNA into cDNA.
Step 3: Make a second strand of DNA.
Step 4: Attach DNA adapters.
Performing Sequencing Reaction
What is the name of the glass plate where we perform the actual sequencing reactions?