This activity will walk you through the sequencing of several short strings of single-stranded DNA (ssDNA). By understanding how sequencing-by-synthesis works at this small scale, it will be easier to picture what's happening with the billions of much longer strings that get sequenced in a real sequencing machine.
First, you will see an example to show you the process. There will be quick questions so you can make sure you understand how it works. Then, you will sequence a flow cell of unknown strings.